Generation of a Large Peptide Phage Display Library by Self-Ligation of Whole-Plasmid PCR Product
نویسندگان
چکیده
منابع مشابه
Puzzling Peptides from a Phage Display Library
The commercial availability of random peptide libraries displayed on the M13 phage is increasing their use forstudies on epitope identification, enzyme inhibitors, receptor ligands, etc. In this study two experimentswhere planned for selection of peptides. First with sheep antibodies, the positive selector was IgG, preparedon Protein G column from a pool of 11 sheeps immunized...
متن کاملidentification of novel targeting peptides for pc3 cells by the screening of a phage display peptide library
background: prostate cancer after lung cancer is the second cause of cancer-associated death in men. in recent years, targeted therapy of cancer has attracted researchers attention. the targeted therapy lead to decrease in side effects of drugs. studies have indicated that targeting peptides for cancer cells represent valuable tools for diagnostic and therapeutic. recently, phage display peptid...
متن کاملGeneration of photoswitchable peptide ligands by phage display
.......................................................................................................................................... iii
متن کاملConstruction of a Nanobodies Phage Display Library From an Escherichia coli Immunized Dromedary
Background: Diarrhea caused by Escherichia coli is a major cause of morbidity and mortality in young animals. Few treatment options are available, mainly antibiotic therapy increasingly limited by resistance to commonly used drugs.Objectives: The aim of this work was to develop immunotherapy based on the use of camel VHH antibody fragments, or nanobodies,...
متن کاملidentification of hitherto undefined b-cell epitopes by antibodies in the sera of vitiligo patients using phage-display peptide library
a random 12 mers phage library was used to screen a pool of immunoglo¬bulin fractions obtained from vitiligo patients. subsequent to panning experiments, a panel of affinity selected phage from vitiligo patients were obtained. this panel was tested using an elis a for their reactivity with pooled sera from patients and normal controls. among the 16 randomly selected clones, two of clones showed...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: ACS Chemical Biology
سال: 2020
ISSN: 1554-8929,1554-8937
DOI: 10.1021/acschembio.0c00497